{"id":968,"date":"2012-04-24T17:04:22","date_gmt":"2012-04-24T17:04:22","guid":{"rendered":"http:\/\/tachinidae.org.uk\/blog\/?p=968"},"modified":"2012-04-25T17:43:21","modified_gmt":"2012-04-25T17:43:21","slug":"the-first-photos-through-the-leica-s8apo","status":"publish","type":"post","link":"https:\/\/tachinidae.org.uk\/blog\/the-first-photos-through-the-leica-s8apo\/","title":{"rendered":"The first photos through the Leica S8APO"},"content":{"rendered":"<p>It has taken a while to get the camera hooked up to the microscope because it is quite a complicated process, involving lots of adapters and converters &#8211; not to mention getting the optics and the extension tubes correctly arranged. The first shots really weren&#8217;t worth looking at but today I started to capture images that are getting a bit better &#8211; still not the quality I hope to achieve, but getting there.<\/p>\n<p>Although the microscope is rated at 80x the actual magnification through the microscope tube is related to the size of the sensor and this works out at about 10x &#8211; 15x but this is still very good and a perfect range for what I want to do. The lighting in these photos is very rough and contrasty because I haven&#8217;t experimented with flash diffusers yet but it gives you an idea of what is possible.<\/p>\n<p>This is a butterfly wing (<em>Eunica coelina<\/em>):<\/p>\n<p><a href=\"http:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Eunica-coelina-s8-2012-04-24-11.05.04-ZS-PMax.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-972\" title=\"Eunica-coelina-s8-2012-04-24-11.05.04-ZS-PMax\" src=\"http:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Eunica-coelina-s8-2012-04-24-11.05.04-ZS-PMax-1024x768.jpg\" alt=\"\" width=\"640\" height=\"480\" srcset=\"https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Eunica-coelina-s8-2012-04-24-11.05.04-ZS-PMax-1024x768.jpg 1024w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Eunica-coelina-s8-2012-04-24-11.05.04-ZS-PMax-300x225.jpg 300w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Eunica-coelina-s8-2012-04-24-11.05.04-ZS-PMax-768x576.jpg 768w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Eunica-coelina-s8-2012-04-24-11.05.04-ZS-PMax-1536x1152.jpg 1536w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Eunica-coelina-s8-2012-04-24-11.05.04-ZS-PMax-150x112.jpg 150w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Eunica-coelina-s8-2012-04-24-11.05.04-ZS-PMax-400x300.jpg 400w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Eunica-coelina-s8-2012-04-24-11.05.04-ZS-PMax.jpg 2048w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/a><\/p>\n<p>This is a lateral view of <em>Dinera grisecens<\/em>, showing a bit of the katepisternum; a nice row of hypopleural bristles; the hind spiracle with hairy flap; haltere and abdominal tergite 1+2:<\/p>\n<p><a href=\"http:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Dinera-grisescens-lateral-s8-2012-04-24-11.53.22-ZS-PMax-2048.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-970\" title=\"Dinera-grisescens-lateral-s8-2012-04-24-11.53.22-ZS-PMax-2048\" src=\"http:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Dinera-grisescens-lateral-s8-2012-04-24-11.53.22-ZS-PMax-2048-1024x768.jpg\" alt=\"\" width=\"640\" height=\"480\" srcset=\"https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Dinera-grisescens-lateral-s8-2012-04-24-11.53.22-ZS-PMax-2048-1024x768.jpg 1024w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Dinera-grisescens-lateral-s8-2012-04-24-11.53.22-ZS-PMax-2048-300x225.jpg 300w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Dinera-grisescens-lateral-s8-2012-04-24-11.53.22-ZS-PMax-2048-768x576.jpg 768w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Dinera-grisescens-lateral-s8-2012-04-24-11.53.22-ZS-PMax-2048-1536x1152.jpg 1536w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Dinera-grisescens-lateral-s8-2012-04-24-11.53.22-ZS-PMax-2048-150x112.jpg 150w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Dinera-grisescens-lateral-s8-2012-04-24-11.53.22-ZS-PMax-2048-400x300.jpg 400w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Dinera-grisescens-lateral-s8-2012-04-24-11.53.22-ZS-PMax-2048.jpg 2048w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/a><\/p>\n<p>Contrast that with the spiracle of Exorista rustica, which has a &#8220;classic&#8221; single-flap arrangement:<\/p>\n<figure id=\"attachment_976\" aria-describedby=\"caption-attachment-976\" style=\"width: 640px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Exorista-rustica-spiracle-s8-2012-04-25-10.36.05-ZS-PMax.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-976\" title=\"Exorista-rustica-spiracle-s8-2012-04-25-10.36.05-ZS-PMax\" src=\"http:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Exorista-rustica-spiracle-s8-2012-04-25-10.36.05-ZS-PMax-1024x768.jpg\" alt=\"\" width=\"640\" height=\"480\" srcset=\"https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Exorista-rustica-spiracle-s8-2012-04-25-10.36.05-ZS-PMax-1024x768.jpg 1024w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Exorista-rustica-spiracle-s8-2012-04-25-10.36.05-ZS-PMax-300x225.jpg 300w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Exorista-rustica-spiracle-s8-2012-04-25-10.36.05-ZS-PMax-768x576.jpg 768w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Exorista-rustica-spiracle-s8-2012-04-25-10.36.05-ZS-PMax-1536x1152.jpg 1536w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Exorista-rustica-spiracle-s8-2012-04-25-10.36.05-ZS-PMax-150x112.jpg 150w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Exorista-rustica-spiracle-s8-2012-04-25-10.36.05-ZS-PMax-400x300.jpg 400w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Exorista-rustica-spiracle-s8-2012-04-25-10.36.05-ZS-PMax.jpg 2048w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/a><figcaption id=\"caption-attachment-976\" class=\"wp-caption-text\">Exorista rustica spiracle - showing the single flap<\/figcaption><\/figure>\n<p>&#8230; and the next 2 show classic polideine spiracles, with 2 equally-sized flaps:<\/p>\n<figure id=\"attachment_978\" aria-describedby=\"caption-attachment-978\" style=\"width: 640px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lypha-dubia-spiracle-s8-2012-04-25-15.23.21-ZS-PMax.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-978\" title=\"Lypha-dubia-spiracle-s8-2012-04-25-15.23.21-ZS-PMax\" src=\"http:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lypha-dubia-spiracle-s8-2012-04-25-15.23.21-ZS-PMax-1024x768.jpg\" alt=\"\" width=\"640\" height=\"480\" srcset=\"https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lypha-dubia-spiracle-s8-2012-04-25-15.23.21-ZS-PMax-1024x768.jpg 1024w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lypha-dubia-spiracle-s8-2012-04-25-15.23.21-ZS-PMax-300x225.jpg 300w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lypha-dubia-spiracle-s8-2012-04-25-15.23.21-ZS-PMax-768x576.jpg 768w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lypha-dubia-spiracle-s8-2012-04-25-15.23.21-ZS-PMax-1536x1152.jpg 1536w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lypha-dubia-spiracle-s8-2012-04-25-15.23.21-ZS-PMax-150x112.jpg 150w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lypha-dubia-spiracle-s8-2012-04-25-15.23.21-ZS-PMax-400x300.jpg 400w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lypha-dubia-spiracle-s8-2012-04-25-15.23.21-ZS-PMax.jpg 2048w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/a><figcaption id=\"caption-attachment-978\" class=\"wp-caption-text\">Lypha dubia spiracle - showing the 2 equally sized flaps<\/figcaption><\/figure>\n<figure id=\"attachment_977\" aria-describedby=\"caption-attachment-977\" style=\"width: 640px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lydina-aenea-spiracle-s8-2012-04-25-11.08.21-ZS-PMax.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-977\" title=\"Lydina-aenea-spiracle-s8-2012-04-25-11.08.21-ZS-PMax\" src=\"http:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lydina-aenea-spiracle-s8-2012-04-25-11.08.21-ZS-PMax-1024x768.jpg\" alt=\"\" width=\"640\" height=\"480\" srcset=\"https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lydina-aenea-spiracle-s8-2012-04-25-11.08.21-ZS-PMax-1024x768.jpg 1024w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lydina-aenea-spiracle-s8-2012-04-25-11.08.21-ZS-PMax-300x225.jpg 300w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lydina-aenea-spiracle-s8-2012-04-25-11.08.21-ZS-PMax-768x576.jpg 768w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lydina-aenea-spiracle-s8-2012-04-25-11.08.21-ZS-PMax-1536x1152.jpg 1536w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lydina-aenea-spiracle-s8-2012-04-25-11.08.21-ZS-PMax-150x112.jpg 150w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lydina-aenea-spiracle-s8-2012-04-25-11.08.21-ZS-PMax-400x300.jpg 400w, https:\/\/tachinidae.org.uk\/blog\/wp-content\/uploads\/Lydina-aenea-spiracle-s8-2012-04-25-11.08.21-ZS-PMax.jpg 2048w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/a><figcaption id=\"caption-attachment-977\" class=\"wp-caption-text\">Lydina aenea spiracle - showing the equally sized flaps<\/figcaption><\/figure>\n<p>I hope to make a few more photos over the coming days, once I have worked out the problem with parfocality &#8211; the camera should be in focus at the same point as the main microscope eyepieces but at the moment it isn&#8217;t &#8230; a bit more experimentation needed! \ud83d\ude09<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>It has taken a while to get the camera hooked up to the microscope because it is quite a complicated process, involving lots of adapters&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[371,369,370,384],"class_list":["post-968","post","type-post","status-publish","format-standard","hentry","category-news","tag-dinera-grisescens","tag-exorista-rustica","tag-lydina-aenea","tag-lypha-dubia"],"_links":{"self":[{"href":"https:\/\/tachinidae.org.uk\/blog\/wp-json\/wp\/v2\/posts\/968","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tachinidae.org.uk\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tachinidae.org.uk\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tachinidae.org.uk\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/tachinidae.org.uk\/blog\/wp-json\/wp\/v2\/comments?post=968"}],"version-history":[{"count":5,"href":"https:\/\/tachinidae.org.uk\/blog\/wp-json\/wp\/v2\/posts\/968\/revisions"}],"predecessor-version":[{"id":982,"href":"https:\/\/tachinidae.org.uk\/blog\/wp-json\/wp\/v2\/posts\/968\/revisions\/982"}],"wp:attachment":[{"href":"https:\/\/tachinidae.org.uk\/blog\/wp-json\/wp\/v2\/media?parent=968"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tachinidae.org.uk\/blog\/wp-json\/wp\/v2\/categories?post=968"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tachinidae.org.uk\/blog\/wp-json\/wp\/v2\/tags?post=968"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}